Glucagon Structure and Function
نویسندگان
چکیده
منابع مشابه
Glucagon structure and function. I. Purification and properties of bovine glucagon and monodesmidoglucagon.
Crystalline bovine or porcine glucagon was fractionated by chromatography on columns of DEAE-cellulose in the presence of buffers containing ‘7 M urea. About 89% of the recovered protein comprises highly purified glucagon; 10% is monodesamidoglucagon and another 0.8 % resembles glucagon in amino acid composition and in electrophoretic mobility. The monodesamidoglucagon was identified and charac...
متن کاملGlucagon Structure and Function I. PURIFICATION AND PROPERTIES OF BOVINE GLUCAGON AND MONODESAMIDOGLUCAGON
Crystalline bovine or porcine glucagon was fractionated by chromatography on columns of DEAE-cellulose in the presence of buffers containing ‘7 M urea. About 89% of the recovered protein comprises highly purified glucagon; 10% is monodesamidoglucagon and another 0.8 % resembles glucagon in amino acid composition and in electrophoretic mobility. The monodesamidoglucagon was identified and charac...
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متن کاملGlucagon structure and function. Preparation and characterization of nitroglucagon and aminoglucagon.
The nitration of glucagon (1%) with tetranitromethane (1 mole excess per mole of tgrosyl residue) proceeded rapidly at pH 8 and 0”, conditions wherein the reactants were almost completely insoluble. Under these conditions few polymeric and oxidative side reactions occurred, and tyrosyl residue 13 reacted much more rapidly than tyrosyl residue 10. The products of nitration were purified by gel f...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45465-3